Journal
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
Volume 109, Issue 4, Pages 543-564Publisher
SPRINGER
DOI: 10.1007/s10482-016-0659-8
Keywords
Botryosphaeriales; Morphology; Phylogeny; Tree pathogens; Urban tree pathology
Categories
Funding
- Tree Protection Co-operative Programme (TPCP)
- University of Pretoria (South Africa)
- Ministry of Education and Science of the Republic of Serbia [TR37008]
- European Cooperation in Science and Technology (COST) Actions Pathway Evaluation in Pest Risk Management In Transport [PERMIT FP1002]
- ALIEN Challenge [TD1209]
- A global network of nurseries as early warning system against alien tree pests (Global Warning) [FP1401]
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Extensive die-back and mortality of various ornamental trees and shrubs has been observed in parts of the Western Balkans region during the past decade. The disease symptoms have been typical of those caused by pathogens residing in the Botryosphaeriaceae. The aims of this study were to isolate and characterize Botryosphaeriaceae species associated with diseased ornamental trees in Serbia, Montenegro, Bosnia and Herzegovina. Isolates were initially characterized based on the DNA sequence data for the internal transcribed spacer rDNA and six major clades were identified. Representative isolates from each clade were further characterized using DNA sequence data for the translation elongation factor 1-alpha, beta-tubulin-2 and large subunit rRNA gene regions, as well as the morphology of the asexual morphs. Ten species of the Botryosphaeriaceae were identified of which eight, i.e., Dothiorella sarmentorum, Neofusicoccum parvum, Botryosphaeria dothidea, Phaeobotryon cupressi, Sphaeropsis visci, Diplodia seriata, D. sapinea and D. mutila were known taxa. The remaining two species could be identified only as Dothiorella spp. Dichomera syn-asexual morphs of D. sapinea, Dothiorella sp. 2 and B. dothidea, as well as unique morphological characters for a number of the known species are described. Based on host plants and geographic distribution, the majority of Botryosphaeriaceae species found represent new records. The results of this study contribute to our knowledge of the distribution, host associations and impacts of these fungi on trees in urban environments.
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