Amycolatopsis xuchangensis sp nov and Amycolatopsis jiguanensis sp nov., isolated from soil

Two novel actinomycetes, designated strains CFH S0322(T) and CFH S01580(T), were isolated from soil samples collected from Xuchang city and Ji Guan cave in Henan province, China, respectively. Their taxonomic positions were investigated by a polyphasic approach. The two isolates were Gram-reaction positive, non-motile, aerobic and catalase-positive actinomycetes. Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the two isolates are closely related to the members of the genus Amycolatopsis, sharing high similarities with Amycolatopsis magusensis KCTC 29056(T) (99.1 %, CFH S0322(T)) and Amycolatopsis nigrescens DSM 44992(T) (98.1 %, CFH S01580(T)), respectively. The major whole cell sugars of strains CFH S0322(T) and CFH S01580(T) were arabinose and galactose and the predominant menaquinone was MK-9(H-4). The genomic DNA G+C contents of strains CFH S0322(T) and CFH S01580(T) were 70.6 and 68.6 mol%, respectively. Morphological, phylogenetic and chemotaxonomic results suggest that strains CFH S0322(T) and CFH S01580(T) are representatives of two new species, for which the names Amycolatopsis xuchangensis sp. nov. and Amycolatopsis jiguanensis sp. nov. are proposed. The type strains are CFH S0322(T) (=NBRC 110769(T) =KCTC 39516(T)) and CFH S01580(T) (=DSM 101526(T) =CCTCC AA 2015032(T)).