Journal
CHEMPLUSCHEM
Volume 84, Issue 7, Pages 816-820Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cplu.201900263
Keywords
fluorescence; FRET; pH sensors; rhodamine; spirolactams
Categories
Funding
- Hong Kong Research Grants Council [HKBU 12300117]
- Hong Kong Baptist University [RC-ICRS-17-18-01]
Ask authors/readers for more resources
A reversible and sensitive pH probe DPE-Rh operates by Forster resonance energy transfer from 1,2-diphenylethyne (DPE) to Rhodamine B (Rh). In the presence of H+, the spirolactam ring of the Rhodamine B unit was opened and this resulted in ca. 1000-fold enhancement of fluorescence intensity with linear change over the pH range of 2.0 to 5.5. The Forster resonance energy transfer offered this probe an effective excitation-emission wavelength shift of around 240 nm with about 100 % quenching of the donor emission. The response of the sensor is tolerant towards a wide range of metal ions and the sensing mechanism was deduced by H-1 NMR spectrometry. This FRET-based molecule not only provides a sensitive pH probe, but also suggests an effective strategy to eliminate the interference of excitation light.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available