Journal
ACS SYNTHETIC BIOLOGY
Volume 8, Issue 6, Pages 1284-1293Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00501
Keywords
alpha-helical coiled coil; de novo protein design; DNA-protein interaction; protein-protein interaction; TAL effectors; transcriptional control
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Funding
- BBSRC through the BrisSynBio Synthetic Biology Research Centre [BB/L01386X1]
- EPSRC through the BrisSynBio Synthetic Biology Research Centre [BB/L01386X1]
- Royal Society Wolfson Research Merit Award [WM140008]
- BBSRC [BB/L01386X/1] Funding Source: UKRI
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An improved ability to direct and control biomolecular interactions in living cells would have an impact on synthetic biology. A key issue is the need to introduce interacting components that act orthogonally to endogenous proteomes and interactomes. Here, we show that low complexity, de novo designed protein protein interaction (PPI) domains can substitute for natural PPIs and guide engineered protein DNA interactions in Escherichia coli. Specifically, we use de novo homo- and heterodimeric coiled coils to reconstitute a cytoplasmic split adenylate cyclase, recruit RNA polymerase to a promoter and activate gene expression, and oligomerize both natural and designed DNA binding domains to repress transcription. Moreover, the stabilities of the heterodimeric coiled coils can be modulated by rational design and, thus, adjust the levels of gene activation and repression in vivo. These experiments demonstrate the possibilities for using designed proteins and interactions to control biomolecular systems such as enzyme cascades and circuits in cells.
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