4.6 Article

Long-term Fiber Photometry for Neuroscience Studies

Journal

NEUROSCIENCE BULLETIN
Volume 35, Issue 3, Pages 425-433

Publisher

SPRINGER
DOI: 10.1007/s12264-019-00379-4

Keywords

Ca2+ signal; Fluorescence detection; Ca2+ indicator; Optical fiber; Experiment protocol

Categories

Funding

  1. Ministry of Science and Technology of China [2012YQ03026005, 2013ZX0950910, 2015BAI08B02]
  2. National Natural Science Foundation of China [91432114, 91632302]
  3. Beijing Municipal Government

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Fiber photometry is a sensitive and easy way to detect changes in fluorescent signals. The combination of fiber photometry with various fluorescent biomarkers has substantially advanced neuroscience research over the last decade. Despite the wide use of fiber photometry in biomedical fields, the lack of a detailed and comprehensive protocol has limited progress and sometimes complicated the interpretation of data. Here, we describe detailed procedures of fiber photometry for the long-term monitoring of neuronal activity in freely-behaving animals, including surgery, apparatus setup, data collection, and analysis.

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