Journal
METHODS IN ECOLOGY AND EVOLUTION
Volume 10, Issue 8, Pages 1128-1135Publisher
WILEY
DOI: 10.1111/2041-210X.13201
Keywords
biodiversity; ecological genetics; environmental DNA; molecular biology; population ecology; sampling; sequencing; species identification
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Funding
- Schmidt Ocean Institute
- Stanford University
- U.S. Environmental Protection Agency (EPA) [F15E211913419]
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Environmental DNA (eDNA) research often requires returning to the laboratory for processing, which can delay species identification by weeks-months. Using a portable Oxford Nanopore MinIon sequencer, eDNA from white shark (Carcharodon carcharias) was rapidly identified from seawater samples collected in the high seas where they have historically been identified with biologging datasets. A total of 10 sequencing runs were performed on the MinIon onboard an oceanographic vessel with a turnaround time from water collection to sequence results and annotation of similar to 48 hr. Identifying vertebrates by amplifying eDNA from seawater provides a novel approach for sampling and detecting the presence of elusive species of conservation importance in remote locations.
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