Journal
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
Volume 60, Issue 7, Pages 4394-4397Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AAC.00444-16
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- University of Fribourg, Fribourg, Switzerland
- ANIHWA-ERA-NET project PRAHAD - Federal Food Safety and Veterinary Office, Bern, Switzerland
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A series of colistin-resistant Escherichia coli clinical isolates was recovered from hospitalized and community patients in South Africa. Seven clonally unrelated isolates harbored the mcr-1 gene located on different plasmid backbones. Two distinct plasmids were fully sequenced, and identical 2,600-bp-long DNA sequences defining a mcr-1 cassette were identified. Promoter sequences responsible for the expression of mcr-1, deduced from the precise identification of the +1 transcription start site for mcr-1, were characterized.
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