Journal
MBIO
Volume 10, Issue 2, Pages -Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/mBio.00128-19
Keywords
B cells; Bifidobacterium; Lactobacillus; core fucosylation; infants; milk N-glycan
Categories
Funding
- National Natural Science Foundation of China [31570797, 31870797]
- Nutrition and Care of Maternal & Child Research Fund Project of Guangzhou Biostime Institute of Nutrition Care [2018BINCMCF39, 2016BINCMCF1102]
- Research Foundation from the Department of Education, Liaoning Province, China [L2016003]
- Liaoning Provincial Program for Top Discipline of Basic Medical Sciences, China
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The maternal milk glycobiome is crucial for shaping the gut microbiota of infants. Although high core fucosylation catalyzed by fucosyltransferase 8 (Fut8) is a general feature of human milk glycoproteins, its role in the formation of a healthy microbiota has not been evaluated. In this study, we found that the core-fucosylated N-glycans in milk of Chinese mothers selectively promoted the colonization of specific gut microbial groups, such as Bifidobacterium spp. and Lactobacillus spp. in their breast-fed infants during lactation. Compared with Fut8(+/-) (WT) mouse-fed neonates, the offspring fed by Fut8(+/+) maternal mice had a distinct gut microbial profile, which was featured by a significant reduction of Lactobacillus spp., Bacteroides spp., and Bifidobacterium spp. and increased abundance of members of the Lachno-spiraceae NK4A136 group and Akkermansia spp. Moreover, these offspring mice showed a lower proportion of splenic CD19(+) CD69(+) B lymphocytes and attenuated humoral immune responses upon ovalbumin (OVA) immunization. In vitro studies demonstrated that the chemically synthesized core-fucosylated oligosaccharides possessed the ability to promote the growth of tested Bifidobacterium and Lactobacillus strains in minimal medium. The resulting L-fucose metabolites, lactate and 1,2-propanediol, could promote the activation of B cells via the B cell receptor (BCR)-mediated signaling pathway. IMPORTANCE This study provides novel evidence for the critical role of maternal milk protein glycosylation in shaping early-life gut microbiota and promoting B cell activation of neonates. The special core-fucosylated oligosaccharides might be promising prebiotics for the personalized nutrition of infants.
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