Journal
ONCOTARGETS AND THERAPY
Volume 12, Issue -, Pages 4203-4211Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/OTT.S196910
Keywords
beta-elemene; radiation; hypoxia; macrophages infiltration; MCP-1; lung cancer
Categories
Funding
- National Natural Science Foundation of China [81703904, 81473452]
Ask authors/readers for more resources
Background: In cancers, tumor-associated macrophages (TAMs) play an important role in the progression, evasion of immunity and sensitivity to therapy. Unfortunately, radiation and hypoxia could induce the M2 macrophages infiltration and polarization. Materials and methods: In this study, we investigated the relevance of macrophage recruitment with radiation and hypoxia by transwell. We also evaluated the effect of beta-elemene on the infiltration of M2 macrophages and explored its underlying molecular mechanism by a series of in vitro and in vivo experiments. Results: Irradiated or hypoxia lung cancer cells recruit macrophages, and the recruitment is MCP-1 dependent. We also found that radiation and hypoxia-induced MCP-1 secretion follows upregulation of Prx-1, which leads to nuclear accumulation of NF-kappa B and HIF-1 alpha expression. In addition, beta-elemene could effectively suppress this recruitment phenomenon through Prx-1/NF-kappa B/HIF-l alpha signaling. Conclusion: Our study showed that radiation and hypoxia significantly promoted the macrophages recruitment. beta-elemene could effectively suppress this recruitment phenomenon and MCP-1 expression via inhibiting Prx-1/NF-kappa B/HIF-1 alpha pathways.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available