Journal
VIRUSES-BASEL
Volume 11, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/v11050455
Keywords
Porcine deltacoronavirus (PDCoV); electron microscopy; ultrastructure; autophagy
Categories
Funding
- National Key Research and Development Program of China [2016YFD0500102]
- National Natural Science Foundation for Young Scientists of China [31802205]
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Porcine deltacoronavirus (PDCoV) causes severe diarrhea and vomiting in affected piglets. The aim of this study was to establish the basic, in vitro characteristics of the life cycle such as replication kinetics, cellular ultrastructure, virion morphology, and induction of autophagy of PDCoV. Time-course analysis of viral subgenomic and genomic RNA loads and infectious titers indicated that one replication cycle of PDCoV takes 5 to 6 h. Electron microscopy showed that PDCoV infection induced the membrane rearrangements with double-membrane vesicles and large virion-containing vacuoles. The convoluted membranes structures described in alpha- and beta-coronavirus were not observed. PDCoV infection also increased the number of autophagosome-like vesicles in the cytoplasm of cells, and the autophagy response was detected by LC3 I/II and p62 Western blot analysis. For the first time, this study presents the picture of the PDCoV infection cycle, which is crucial to help elucidate the molecular mechanism of deltacoronavirus replication.
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