SNP discovery in radiata pine using a de novo transcriptome assembly

Key messageA set of single-nucleotide polymorphisms (SNPs) was discovered using a de novo transcriptome for radiata pine. The reference transcriptome and SNPs were annotated according to the functional information available in biological databases.A set of SNPs was discovered using a de novo transcriptome assembly for radiata pine in Chile. De novo transcriptome was generated by Illumina sequences and assembled by Trinity. A set of unique genes and transcription factors were identified. All dataset sequences were mapped against the de novo reference transcriptome and SNPs were called. A total of 300,761 SNPs were identified between samples, where 38,730 SNPs had a functional annotation. Since there is little data available of radiata pine-omics, the aim of this work is to provide to the scientific community a de novo transcriptome assembly and a set of SNPs.