Journal
TETRAHEDRON
Volume 75, Issue 27, Pages 3722-3732Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tet.2019.05.044
Keywords
Light-up bioprobes; Tetraphenylethylene (TPE); Aggregation-induced emission (AIE); Water-soluble; Protein and DNA binding; Wash-free bacterial imaging
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Funding
- DST-SERB, India [EMR/2016/001416, EMR/2016/002253]
- CSIR, India
- DST-SERB
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Available online Development of water soluble AIE-active light-up bioprobes for the detection of biomacromolecules has drawn huge research interests in recent past. In this study, a series of ethylene glycol modified water soluble tetrameric tetraphenylethylene amphiphiles with pyridinium polar heads (TPE-xEG-Py, x = 3, 4, 6 or 1a-c) have been synthesized by varying the ethylene glycol spacer. Their unique structure allows them to form vesicles and other nanoaggregates in aqueous solutions. These amphiphiles were successfully utilized for fluorimetric detection and quantitation of BSA and DNA based on the electrostatic interactions to trigger AIE-emission from the TPE moiety. The electrostatic interaction was also proved very effective in wash-free imaging of both Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria with up to 92 folds increase in fluorescence response within bacterial concentration 0-12 x 10(8) CFU mL(-1). The strategy is advantageous due to cost-effective and easy synthesis, high water solubility, and fast response. (C) 2019 Elsevier Ltd. All rights reserved.
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