Journal
TALANTA
Volume 196, Issue -, Pages 145-152Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.12.031
Keywords
Fluorescent probe; Biothiols; Long-wavelength-emitting; Cell imaging
Categories
Funding
- NSFC [21572209]
- Program for Innovative Research Team (in Science and Technology) in University of Henan Province [17IRTSTHN002]
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A long-wavelength fluorescent probe NR-CY was developed for simultaneous identification of cysteine/glutathione and sulphide by combining the derivative of Nile red with 7-nitrobenzofurazan. The response of NR-CY to thiols is regulated by intramolecular charge transfer and photoinduced electron transfer mechanisms. For sulphide at 560 nm, cysteine at 475 nm and glutathione at 425 nm, different absorbance increases can be observed. NR-CY can detect cysteine at fluorescence emission 543 nm and distinguish sulphide from other analytes by kinetic experiments at 636 nm. The probe showed a rapid response to these thiols (cysteine was 90 s and sulphide was 30 s). In addition, NR-CY has been successfully applied to live MCF-7 cell imaging.
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