Journal
TALANTA
Volume 197, Issue -, Pages 270-276Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2019.01.023
Keywords
Black phosphorus nanosheets; Perylene probe; Protease; Fluorescence; Inhibitor; Trypsin
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Funding
- National Natural Science Foundation of China [21561162004, 51761145102, 21874128]
- Jilin Provincial Science and Technology Development Project (International Collaboration Program) [20160414040GH]
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Proteases, as one of the most significant kind of digestive enzymes, are closely related to a variety of physiological processes and diseases. Herein, a black phosphorus nanosheets (BPs) based sensitive fluorometric method for protease detection and inhibitor screening is proposed. The aqueous solution of perylene probe (probe 1) displays a strong fluorescence. BPs, as novel discovered two-dimensional (2D) materials, can adsorb probe 1 through electrostatic interactions, which causes fluorescence quenching of probe 1. Histone can control the interactions between the perylene probe and BPs, which can be further regulated by the introduction of a protease. Thus, the protease activity can be monitored by detecting the fluorescence intensity changes of probe 1. The method is label free, sensitive and selective. As low as 1 ng/mL trypsin can be easily detected.
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