Journal
TALANTA
Volume 195, Issue -, Pages 655-661Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.11.110
Keywords
3-nitrotyrosine; Surface plasmon resonance (SPR); ELISA; Human urine
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Funding
- Guangzhou Science and Technology Foundation [2016201604030025]
- Guangdong Science and Technology Foundation [2017A050501034]
- National Research Technical University MISiS [K3-2017-073]
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3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this paper, on the basis of anti-3-NT specific antibody, we developed a label-free indirect competitive surface plasmon resonance (SPR) immunosensor and ELISA for the detection of 3-NT. Under the optimized conditions, the SPR immunosensor can obtain a linear range of 0.17-6.07 mu g/mL and a limit of detection (LOD) of 0.12 mu g/mL while the ELISA can reach 0.33-9.94 mu g/mL and a LOD of 0.24 mu g/mL.The selectivity of 3-NT was also testified by six kinds of amino acid analogues. Besides, the developed SPR immunosensor was compared thoroughly with a conventional ELISA in spiked analysis of urine samples. Good recoveries and correlation between these two methods were observed (R-2 = 0.964). Therefore, it is concluded that the automated SPR platform can be applied to quantify 3-NT in biological samples with its sensitivity, accuracy, and real timing.
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