Journal
TALANTA
Volume 195, Issue -, Pages 333-338Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2018.11.073
Keywords
Paper-based devices; Norfloxacin; Milk; Fluorescent immunoassay; Quantum dots
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Funding
- National Natural Science Foundation of China [21675085, 81672508, 61505076, 61505077, 21505072]
- National Key R&D Program of China [2017YFA0204700]
- China-Sweden Joint Mobility Project [51661145021]
- Natural Science Foundation of Jiangsu Province for Distinguished Young Scholars [BK20170042, BK20170041]
- Key University Science Research Project of Jiangsu Province [16KJA180004]
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Norfloxacin (NOR) in milk may influence mammalian cell replication and bring about a decrease in the efficiency for treating infection in humans. However, current techniques for detecting NOR usually require expensive instruments and trained personnel. In this work, we have developed a low-cost and simple method via paper-based fluorescent immunoassay for highly sensitive and selective detection of NOR in milk at picogram level. The NOR monoclonal antibody labeled with quantum dots is used as a detection probe to recognize the corresponding NOR, which can quantitatively detect NOR on paper-based devices. The detection limits in aqueous solution and milk are 1 pg/mL and 10 pg/mL, respectively. The developed paper-based method provides a cheap, sensitive, eco-friendly, and rapid approach for quantitative detection of trace NOR in milk, which may find wide applications in food safety inspection. Noteworthy, the method is especially suitable for applications at resource-limited and on-site settings.
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