Journal
SMALL
Volume 15, Issue 19, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201900735
Keywords
aptamers; electrochemical; gastric cancer exosomes; rolling circle amplification
Categories
Funding
- National Key Research and Development Program of China [2017YFA0205301]
- National Natural Scientific Foundation of China [61741114, 61527806]
- Jiangsu Province Medical Talent [ZDRCA2016065]
- Medical Key Science and Technology Development Projects of Nanjing [ZKX16045]
- Science and Technology Development Program of Nanjing [201715020]
- Health Care for Cadres Research Fund of Jiangsu Province [BJ16003]
- State Key Laboratory of Bioelectronics [201705]
- New Technology Introduction and Development Fund of Nanjing Drum Tower Hospital [xjsyjfzjj201615]
- Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province [(2013) 448]
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Emerging evidence indicates that exosomes derived from gastric cancer cells enhance tumor migration and invasion through the modulation of the tumor microenvironment. However, it remains a major problem to detect cancer-specific exosomes due to technical and biological challenges. Most of the methods reported could not achieve efficient detection of tumor-derived exosomes in the background of normal exosomes. Herein, a label-free electrochemical aptasensor is presented for specific detection of gastric cancer exosomes. This platform contains an anti-CD63 antibody modified gold electrode and a gastric cancer exosome specific aptamer. The aptamer is linked to a primer sequence that is complementary to a G-quadruplex circular template. The presence of target exosomes could trigger rolling circle amplification and produce multiple G-quadruplex units. This horseradish peroxidase mimicking DNAzyme could catalyze the reduction of H2O2 and generate electrochemical signals. This aptasensor exhibits high selectivity and sensitivity toward gastric cancer exosomes with a detection limit of 9.54 x 10(2) mL(-1) and a linear response range from 4.8 x 10(3) to 4.8 x 10(6) exosomes per milliliter. Therefore, this electrochemical aptasensor is expected to become a useful tool for the early diagnosis of gastric cancer.
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