Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 284, Issue -, Pages 73-80Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.12.126
Keywords
Bisphenol A; Aptasensor; Disposable; Single-stranded DNA; Methylene blue
Funding
- National Science Foundation of China [21527808, 21625501]
- Dean Foundation of Chinese Academy of Inspection and Quarantine [2017JK041]
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A disposable and sensitive electrochemical aptasensor was developed based on a single-stranded DNA (ssDNA)-methylene blue (MB) complex for the determination of bisphenol A (BPA). BPA-specific aptamer, a ssDNA with hairpin structure, was immobilized on a low-cost plastic electrode and acted as both the bio-recognition element and the signal-amplification platform. MB was then intercalated into the aptamer and acted as the signaling probe. The intercalated MB was liberated upon interaction with BPA due to DNA conformational change, thereby resulting in the reduction of electrochemical response. The proposed sensors in the intermediate and final stages were carefully characterized. Under optimal conditions, a good linear relationship was presented with the logarithm of BPA concentrations in the range of 0.001-100 mu g/L, and a detection limit of 0.4 ng/L was obtained. This proposed sensor was applied to detect BPA in real samples and achieved satisfying results.
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