Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 285, Issue -, Pages 76-83Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.01.041
Keywords
Biocompatibility; Intra-molecular charge transfer (ICT); Large stokes shift; Mitochondria selectivity; Styryl dye; Wash-free application
Funding
- NIH CBBI fellowship [T32GM075762]
- Indiana University School of Medicine-South Bend Imaging and Flow Cytometry core
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Bright red-emitting pyridinium cyanine based styryl probe 2 is synthesized in good yields. Probe 2 demonstrated a large Stokes' shift (Lambda lambda approximate to 128 nm, 4227 cm(-1) in DCM) and excellent fluorescent quantum yield (Phi(f1) approximate to 0.2 - 0.7) due to strong Intra-molecular charge transfer (ICT). Probe 2 was found to exhibit exceptional selectivity for cellular mitochondria in both normal (COS-7) and cancer (A549) cell lines. Probe 2 is readily applicable as a wash-free dye to visualize mitochondria as it does not require post-staining washing prior to imaging. Styryl probe 2 also showed an excellent biocompatibility as the calculated LC50 (lethal concentration, 50%) value was > 20 mu M. Probe 2 emission did not show any interferences from anionic species or other biological molecules. Probe 2 is readily excitable (lambda(ex)similar to 460 and lambda(em)similar to 618 nm) with the available laser (454 nm) in commercial microscopes and thus it can be a useful probe for mitochondrial tracking in live cells.
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