Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 296, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.048
Keywords
Fluorescent probe; Large stokes-shifted; Excited-state intramolecular proton transfer (ESIPT); Intramolecular charge transfer (ICT); Near-infrared (NIR); Cysteine
Funding
- National Natural Science Foundation of China [21405057, 21207047]
- Science and Technology Developing Foundation of Jilin Province of China [20180201050YY, 201701011106JC]
- Industrial Innovation Funds of Jilin Province of China [2018C034-1]
Ask authors/readers for more resources
Water solubility, large Stokes-shift, and near-infrared emission are essential properties required for a fluorescent probe used for intracellular detection of the analytes. Herein, we report a new fluorescent probe, BMQA, obtained from the reaction of 2-(2-hydroxyphenyl) benzothiazole, methylquinoline, and acryloyl chloride. By using this probe, it is possible to quantitatively detect cysteine (Cys) in 1% acetonitrile-containing PBS buffer solution (pH 7.2). The experimental results showed that the probe exhibited an excellent spectral performance with an emission wavelength at 740 nm, Stokes shift of 310 nm, and detection limit of 0.062 mu M for Cys. Theoretical calculations were also conducted to clarify the luminescence mechanism of the system. In addition, the experiments performed on A549 living cells showed that BMQA is suitable for the in detection of Cys in human serum by fluorescence imaging.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available