Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 284, Issue -, Pages 437-443Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2018.12.149
Keywords
Mesoporous silica; Gold nanoparticles; Electrochemiluminescence; Biochip array; Reactive oxygen species
Funding
- National Natural Science Foundation of China [21505159, 81673569]
- 111 Project [B16046]
- State Key Laboratory of Analytical Chemistry for Life Science, Nanjing University [SKLACLS1714]
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Au nanoparticles (NPs) has been widely used for the detection of intracellular H2O2 to enhance the electron transfer process. But AuNPs are easy to aggregate in the live cells environment. Herein we report a rapid, reliable and low-cost electrochemiluminescent (ECL) biochip integrated by AuNPs-loaded mesoporous silica film (MSF) to detect H2O2 released by macrophage cells. The MSF was employed as a template to load AuNPs within the nanochannels to avoid aggregation. H2O2 could be catalyzed by AuNPs to promote the ECL reaction of luminol molecules in solution. The ECL intensity was significantly enhanced, and the peak potential was negatively shifted by 400 mV due to the excellent electrocatalytic ability of AuNPs. The integrated biochip demonstrated good reproducibility, with a wide linear range of 0.1-200 mu M and an LOD of 25.3 nM. The reliability was evaluated by applying for the assessment of antioxidant activity of resveratrol using RAW 264.7 macrophage model. The AuNPs-loaded MSF integrated biochip can be easily adapted to the development of improved devices in biosensing, lab-on-a-chip, and nanofluidic systems.
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