Journal
RNA BIOLOGY
Volume 16, Issue 9, Pages 1147-1155Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/15476286.2019.1621121
Keywords
Gene expression; human cells; cancer; coupling; mRNA decay; transcription; translation; regulation
Categories
Funding
- Coventry University
- Centre for Sports, Exercise and Life Sciences, Coventry University
Ask authors/readers for more resources
Evidence from yeast and mammals argues the existence of cross-talk between transcription and mRNA decay. Stabilization of transcripts upon depletion of mRNA decay factors generally leads to no changes in mRNA abundance, attributing this to decreased transcription rates. We show that knockdown of human XRN1, CNOT6 and ETF1 genes in HepG2 cells led to significant alteration in stability of specific mRNAs, alterations in half-life were inversely associated with transcription rates, mostly not resulting in changes in abundance. We demonstrate the existence of the gene expression buffering mechanism in human cells that responds to both transcript stabilization and destabilization to maintain mRNA abundance via altered transcription rates and may involve translation. We propose that this buffering may hold novel cancer therapeutic targets.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available