Journal
RADIATION PHYSICS AND CHEMISTRY
Volume 167, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.radphyschem.2019.04.060
Keywords
Cortical bone; Demineralization; Small angle X-ray scattering; X-ray microfluorescence; Synchrotron microtomography
Funding
- Brazilian National Synchrotron (LNLS-Project) [IMX-20180017]
- CNPq
- FAPERJ
- FINEP [CT-INFRA 01.13.0444.02]
- CAPES [001]
Ask authors/readers for more resources
The aim of this study is to compare the composition and structure of cortical bones with different stages of in-vitro demineralization from the micro (10-500 mu m) to the nano-scale level (below 1 mu m) using X-ray techniques, such as Small Angle X-ray Scattering (SAXS), X-ray Microfluorescence (mu-XRF) and Synchrotron Microtomography (SR-mu CT). In total, 8 fresh cortical bone samples of different ages from 2 diaphyseal femora were selected. They were submitted to a 4 days demineralization experiment with 2 concentrations of EDTA acid (0.1 mol L-1 pH 10, and 0.5 mol L-1 pH 7.4, at 25 degrees C, without agitation). EDTA is a slow demineralization agent and permits better tissue preservation of bone specimens. mu-XRF elemental mapping showed that EDTA 0.1 mol L-1 demineralized more homogeneously the samples than EDTA 0.5 mol.L-1 (pH 7.4). SR-mu CT data have demonstrated the changes that occurred in the 3D internal microstructure of cortical bone during the demineralization and besides the results concluded that both EDTA concentrations had similar effect on the intracortical porosity. From SAXS analysis EDTA 0.1 mol L-1 had a better performance in the demineralization of the samples without damaging the nanostructures.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available