Journal
BIOMACROMOLECULES
Volume 16, Issue 4, Pages 1322-1330Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.biomac.5b00113
Keywords
-
Funding
- National Natural Science Foundation of China [NSFC 51273137, 51273139, 51473110]
- National Science Fund for Distinguished Young Scholars [NSFC 51225302]
- Jiangsu Specially-Appointed Professor Program
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Ask authors/readers for more resources
pH-Responsive chimaeric polypeptide-based polymersomes (refer to as pepsomes) were designed and developed from asymmetric poly(ethylene glycol)-b-poly(L-leucine)-b-poly(L-glutamic acid) (PEG-PLeu-PGA, PEG is longer than PGA) triblock copolymers for efficient encapsulation and triggered intracellular delivery of doxorubicin hydrochloride (DOX center dot HCl). PEG-PLeu-PGA was conveniently prepared by sequential ring-opening polymerization of L-leucine N-carboxyanhydride and gamma-benzyl-L-glutamate N-carboxyanhydride using PEG-NH2 as an initiator followed by deprotection. Pepsomes formed from PEG-PLeu-PGA had unimodal distribution and small sizes of 64-71 nm depending on PLeu block lengths. Interestingly, these chimaeric pepsomes while stable at pH 7.4 were quickly disrupted at pH 5.0, likely due to alternation of ionization state of the carboxylic groups in PGA that shifts PGA blocks from hydrophilic and random coil structure into hydrophobic and a-helical structure. DOX center dot HCl could be actively loaded into the watery core of pepsomes with a high loading efficiency. Remarkably, the in vitro release studies revealed that release of DOX center dot HCl was highly dependent on pH, in which about 24.0% and 75.7% of drug was released at pH 7.4 and 5.0, respectively, at 37 degrees C in 24 h. MTT assays demonstrated that DOX center dot HCl-loaded pepsomes exhibited high antitumor activity, similar to free DOX center dot HCl in RAW 264.7 cells. Moreover, they were also potent toward drug-resistant MCF-7 cancer cells (MCF-7/ADR). Confocal microscopy studies showed that DOX center dot HCl-loaded pepsomes delivered and released drug into the cell nuclei of MCF-7/ADR cells in 4 h, while little DOX center dot HCl fluorescence was observed in MCF-7/ADR cells treated with free drug under otherwise the same conditions. These chimaeric pepsomes with facile synthesis, efficient drug loading, and pH-triggered drug release behavior are an attractive alternative to liposomes for targeted cancer chemotherapy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available