Journal
PLANT PHYSIOLOGY AND BIOCHEMISTRY
Volume 141, Issue -, Pages 133-141Publisher
ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
DOI: 10.1016/j.plaphy.2019.05.029
Keywords
Plant cell cultures; Root-rhizome cultures; Ruscus aculeatus; Ruscogenin biosynthesis
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Funding
- Spanish Ministerio de Ciencia, Innovacion y Universidades [BIO2017-82374-R]
- AEI/FEDER, UE [BIO2017-82374-R]
- University Pompeu Fabra
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Ruscus aculeatus is a threatened medicinal plant whose main bioactive components, the ruscogenins, have long been used in the treatment of hemorrhoids and varicose veins, but recently demonstrated activity against some types of cancer. Plant cell biofactories could constitute an alternative to the whole plant as a source of ruscogenins. In this pipeline, despite the in vitro recalcitrance of R. aculeatus, after many attempts we developed friable calif and derived plant cell suspensions, and their ruscogenin production was compared with that of organized in vitro plantlet and root-rhizome cultures. Root-rhizomes showed a higher capacity for biomass and ruscogenin production than the cell suspensions and the yields were greatly improved by elicitation with coronatine. Although ruscogenins accumulate in plants mainly in the root-rhizome, it was demonstrated that the aerial part could play an important role in their biosynthesis, as production was higher in the whole plant than in the root-rhizome cultures.
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