Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 158, Issue -, Pages 12-17Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2019.04.001
Keywords
ALS; Gene mutation; Herbicide resistance; Lithospermum arvense
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Funding
- National Natural Science Foundation of China [31772181, 31601653]
- Project of Shandong Province Higher Educational Science and Technology Program [J18KA134]
- National Key R&D Program of China [2016YFD0300709, 2016YFD0300701]
- Funds of Shandong Double Tops Program [SYL2017XTTD11]
- Major Science and Technology Innovation Project in Shandong Province [2018CXGCO213]
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Lithospermum arvense is a troublesome dicotyledonous winter annual weed of wheat in China. A L. arvense population (HNO1) suspected of being resistant to acetolactate synthase (ALS) inhibitors was found in Henan Province, China. This study aimed to testify the sensitivity of this HNO1 population to eight herbicides from 3 different modes of action, and to explore the potential target-site-resistance mechanism to tribenuron-methyl. The whole-plant bioassays indicated that the population was highly resistant to tribenuron-methyl (SU, 350 fold), pyrithiobac sodium (PTB, 151-fold), pyroxsulam (TP, 62.7-fold), florasulam (TP, 80.6-fold), and imazethapyr (IMI, 136-fold), but was sensitive to carfentrazone-ethyl and fluroxypyr-meptyl. ALS gene sequencing revealed that the Trp (TGG) was substituted by Leu (TTG) at codon 574 in resistant plants. In in vitro ALS assays, the concentration of tribenuron-methyl required to inhibit 50% ALS activity (I-50) for HN01 was 117-fold greater than that required to inhibit a susceptible population (HN05), indicating that resistance was due to reduced sensitivity of the ALS enzyme to tribenuron-methyl. To the best of our knowledge, this is the first report of ALS gene Trp-574-Leu amino acid mutation confer resistance to tribenuron-methyl in L. arvense.
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