Journal
OPTICS AND LASERS IN ENGINEERING
Volume 115, Issue -, Pages 238-242Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.optlaseng.2018.12.001
Keywords
Digital holographic microscopy; Aberration compensation; Phase measurement
Categories
Funding
- National Natural Science Foundation of China (NSFC) [61471313]
- Natural Science Foundation of Hebei Province [F2018203229]
- Qinhuangdao Science and Technology Bureau [201703A009]
- Yanshan University Doctoral Funds [B1025]
Ask authors/readers for more resources
We propose a purely automatic and accurate phase aberration compensation method in digital holographic microscopy for phase-contrast imaging of living cells. At first, a least-squares fitting procedure is implemented to correct a large proportion of the phase aberrations. Subsequently, the flat phase background is retrieved based on phase gradient and image segmentation. Finally, the residual phase aberrations are eliminated in another least-squares fitting procedure performed within the background region. This method needs no manual intervention or prior knowledge of optical setup. Not only phase curvature but also high order aberrations could be corrected without extra components. It outperforms the phase-variation-minimization method in terms of noise robustness and calculation speed. The performance of the proposed method is demonstrated with simulated and experimental results.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available