Journal
NUCLEIC ACIDS RESEARCH
Volume 47, Issue 11, Pages 5867-5879Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz243
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Funding
- Deutsche Forschungsgemeinschaft [SFB 958 A21, TRR 186 A16]
- German Research Foundation
- Open Access Publication Fund of the Freie Universitat Berlin
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In the yeast U1 snRNP the Prp39/Prp42 heterodimer is essential for early steps of spliceosome assembly. In metazoans no Prp42 ortholog exists, raising the question how the heterodimer is functionally substituted. Here we present the crystal structure of murine PRPF39, which forms a homodimer. Structure-guided point mutations disrupt dimer formation and inhibit splicing, manifesting the homodimer as functional unit. PRPF39 expression is controlled by NMD-inducing alternative splicing in mice and human, suggesting a role in adapting splicing efficiency to cell type specific requirements. A phylogenetic analysis reveals coevolution of shortened U1 snRNA and the absence of Prp42, which correlates with overall splicing complexity in different fungi. While current models correlate the diversity of spliceosomal proteins with splicing complexity, our study highlights a contrary case. We find that organisms with higher splicing complexity have substituted the Prp39/Prp42 heterodimer with a PRPF39 homodimer.
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