Journal
NUCLEIC ACIDS RESEARCH
Volume 47, Issue 11, Pages 5502-5510Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz215
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Funding
- Agence Nationale de la Recherche [ANR-17-CE17-0010-01]
- European Research Council [H2020-MSCA-IF-2016-750368]
- Pharmacoimagerie & agents theranostiques project - Universite de Bourgogne
- Conseil Regional de Bourgogne (PARI)
- European Union (PO FEDER-FSE Bourgogne 2014/2020 programs)
- Agence Nationale de la Recherche (ANR) [ANR-17-CE17-0010] Funding Source: Agence Nationale de la Recherche (ANR)
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Guanine-rich DNA and RNA sequences can fold into higher-order structures known as G-quadruplexes (or G4-DNA and G4-RNA, respectively). The prevalence of the G4 landscapes in the human genome, transcriptome and ncRNAome (non-coding RNA), collectively known as G4ome, is strongly suggestive of biological relevance at multiple levels (gene expression, replication). Small-molecules can be used to track G4s in living cells for the functional characterization of G4s in both normal and disease-associated changes in cell biology. Here, we describe biotinylated biomimetic ligands referred to as BioTASQ and their use as molecular tools that allow for isolating G4s through affinity pull-down protocols. We demonstrate the general applicability of the method by purifying biologically relevant G4s from nucleic acid mixtures in vitro and from human cells through the G4RP-RT-qPCR protocol. Overall, the results presented here represent a step towards the optimization of G4-RNAs identification, a key step in studying G4s in cell biology and human diseases.
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