Journal
NATURE METHODS
Volume 16, Issue 6, Pages 553-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41592-019-0400-4
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Funding
- Swiss National Science Foundation [310030B_170269]
- European Research Council (ERC Advanced Grant) [670757]
- SystemsX.ch [51TP-0_145729, 51PHP0_157359]
- Roche Joint Collaborative Project
- European Research Council (ERC) [670757] Funding Source: European Research Council (ERC)
- Swiss National Science Foundation (SNF) [310030B_170269] Funding Source: Swiss National Science Foundation (SNF)
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Animal behavior originates from neuronal activity distributed across brain-wide networks. However, techniques available to assess large-scale neural dynamics in behaving animals remain limited. Here we present compact, chronically implantable, high-density arrays of optical fibers that enable multi-fiber photometry and optogenetic perturbations across many regions in the mammalian brain. In mice engaged in a texture discrimination task, we achieved simultaneous photometric calcium recordings from networks of 12-48 brain regions, including striatal, thalamic, hippocampal and cortical areas. Furthermore, we optically perturbed subsets of regions in VGAT-ChR2 mice by targeting specific fiber channels with a spatial light modulator. Perturbation of ventral thalamic nuclei caused distributed network modulation and behavioral deficits. Finally, we demonstrate multi-fiber photometry in freely moving animals, including simultaneous recordings from two mice during social interaction. High-density multi-fiber arrays are versatile tools for the investigation of large-scale brain dynamics during behavior.
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