Journal
MOLECULES
Volume 24, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/molecules24081610
Keywords
Lentinus edodes; orthogonal optimization; (16)--d-glucan; structure characterization; solution conformation; antioxidant; immunomodulatory activities
Funding
- National Science and Technology Major Project of China Significant New Drugs Development [2018ZX09735004]
- National Natural Science Foundation of China
- NSFC-Shandong Joint Fund for Marine Science Research Centers [31670811, 21602212, U1606403]
- Fundamental Research Funds for the Central Universities [201762002]
- National Key Research and Development Program of China [2018YFC0310900]
- Basic Research Funds for Application of Qingdao [17-1-1-63-jch]
- Major Science and Technology Projects in Shandong Province [2015ZDJS04002]
- Shandong Provincial Key Laboratory of Glycoscience Industry Alliance
- Qingdao Scientific and Technological Innovation Center for Marine Biomedicine Development Grant
- Taishan Scholar Project Special Funds
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The purpose of this study is to develop a robust approach to obtain glucans from Lentinus edodes and to characterize their structural and biological properties for sustainable utilization. The alkali extraction was optimized with an orthogonal experimental design, and a concise process for obtaining specific targeting polysaccharides from Lentinus edodes was developed in this study. After purification with a Q-Sepharose Fast Flow strong anion-exchange column, the monosaccharide composition, a methylation analysis, and NMR spectroscopy were employed for their structural characterizations. LeP-N2 was found to be composed of (16)--d-glucans with minor -(13) glucosidic side chains. Atomic force microscopy (AFM) and high-performance gel permeation chromatography-refractive index-multi-angle laser light scattering (HPGPC-RI-MALLS) also revealed LeP-N2 exhibiting a compact unit in aqueous solution. This (16)--d-glucan was tested for antioxidant activities with IC50 at 157 g/mL. Moreover, RAW 264.7 macrophage activation indicated that the release of nitric oxide (NO) and reactive oxygen species (ROS) were markedly increased with no cytotoxicity at a dose of 100 g/mL. These findings suggest that the (16)--d-glucans obtained from Lentinus edodes could serve as potential agents in the fields of functional foods or medicine.
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