Journal
MOLECULES
Volume 24, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/molecules24091687
Keywords
Ranae Oviductus; identification; protein; RP-HPLC; fingerprint
Funding
- Administration of Traditional Chinese Medicine of Jilin Province [DBXM085-2018]
- Jilin Bureau of Quality and Technical Supervision [2019037]
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This work demonstrated a method combining reversed-phase high-performance liquid chromatography (RP-HPLC) with chemometrics analysis to identify the authenticity of Ranae Oviductus. The fingerprint chromatograms of the Ranae Oviductus protein were established through an Agilent Zorbax 300SB-C8 column and diode array detection at 215 nm, using 0.085% TFA (v/v) in acetonitrile (A) and 0.1% TFA in ultrapure water (B) as mobile phase. The similarity was in the range of 0.779-0.980. The fingerprint chromatogram of Ranae Oviductus showed a significant difference with counterfeit products. Hierarchical clustering analysis (HCA) and principal component analysis (PCA) successfully identified Ranae Oviductus from the samples. These results indicated that the method established in this work was reliable.
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