Journal
MOLECULAR PLANT
Volume 12, Issue 9, Pages 1259-1277Publisher
CELL PRESS
DOI: 10.1016/j.molp.2019.05.007
Keywords
mTERF5; transcriptional pausing; transcription; chloroplast; psbEFLJ
Categories
Funding
- National Natural Science Foundation of China [31730102]
- State Key Basic Research and Development Plan of China [2015CB150105]
- Key Research Plan of Frontier Sciences of the Chinese Academy of Sciences [QYZDJ-SSW-SMC003]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDB17030100]
- National Key Scientific Instrument and Equipment Development Project of China [2013YQ030595]
Ask authors/readers for more resources
RNA polymerase transcriptional pausing represents a major checkpoint in transcription in bacteria and metazoans, but it is unknown whether this phenomenon occurs in plant organelles. Here, we report that transcriptional pausing occurs in chloroplasts. We found that mTERF5 specifically and positively regulates the transcription of chloroplast psbEFLJ in Arabidopsis thaliana that encodes four key subunits of photo-system II. We found that mTERF5 causes the plastid-encoded RNA polymerase (PEP) complex to pause at psbEFLJ by binding to the +30 to +51 region of double-stranded DNA. Moreover, we revealed that mTERF5 interacts with pTAC6, an essential subunit of the PEP complex, although pTAC6 is not involved in the transcriptional pausing at psbEFLJ. We showed that mTERF5 recruits additional pTAC6 to the transcriptionally paused region of psbEFLJ, and the recruited pTAC6 proteins could be assembled into the PEP complex to regulate psbEFLJ transcription. Taken together, our findings shed light on the role of transcriptional pausing in chloroplast transcription in plants.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available