Journal
MOLECULAR BIOLOGY REPORTS
Volume 46, Issue 3, Pages 3451-3460Publisher
SPRINGER
DOI: 10.1007/s11033-019-04808-5
Keywords
Aspergillus capensis; Plant endophyte; Antifungal metabolites; Penicillither; Methyl dichloroasterrate; Rosellichalasin
Categories
Funding
- China Agriculture Research System [CARS-12]
- National Key Research and Development Program of China [2018YFD0200906]
Ask authors/readers for more resources
An isolate (CanS-34A) of Aspergillus from a healthy plant of oilseed rape (Brassica napus) was identified based on morphological characterization and multi-locus phylogeny using the sequences of internal transcribed spacer (ITS)-5.8S rDNA region, BenA (for -tubulin), CaM (for calmodulin) and RPB2 (for RNA polymerase II). The results showed that CanS-34A belongs to Aspergillus capensis Hirooka et al. The antifungal metabolites produced by CanS-34A in potato dextrose broth (PDB) were extracted with chloroform. Three antifungal metabolites were isolated and purified from the chloroform extract of the PDB cultural filtrates of CanS-34A, and chemically identified as methyl dichloroasterrate, penicillither and rosellichalasin. They all showed antifungal activity against the plant pathogenic fungi Botrytis cinerea, Monilinia fructicola, Sclerotinia sclerotiorum and Sclerotinia trifoliorum with the EC50 values ranging from 2.46 to 65.00g/mL. To our knowledge, this is the first report about production of penicillither by Aspergillus and about the antifungal activity of methyl dichloroasterrate, penicillither and rosellichalasin against the four plant pathogenic fungi.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available