Journal
MICROCHIMICA ACTA
Volume 186, Issue 6, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3472-z
Keywords
Biomarker; Biosensor; Aptamer; PEG 600; Myocardial infarction; Redox marker; Acute coronary syndromes; Myocyte damage; Nanogold; Macropore
Categories
Funding
- Research Councils of Shiraz University of Medical Sciences [14588]
- Shahid Sadoughi University of Medical Sciences
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A specific troponin T (TnT) binding aptamer was identified and immobilized on an electrodeposited macroporous gold nanostructure using poly(ethylene glycol) 600, to fabricate a novel and ultrasensitive TnT aptasensor. The transducer surface on the gold disk electrode was characterized by field emission scanning electron microscopy, and immobilization of the aptamer was monitored by open circuit potential measurements. Binding of TnT by the aptamer was monitored by differential pulse voltammetry using ferro/ferricyanide as the redox probe. The aptamer has a high affinity and specificity, and the electrode is sensitive and selective. Best operated at a working potential of 0.23V (vs. Ag/AgCl), the electrode can detected TnT in the 0.05 to 5.0ngmL(-1) concentration range with a 23pgmL(-1) detection limit. The method was applied to the determination of TnT in 99 spiked human serum samples, and the diagnostic sensitivity and specificity were 94 and 95%, respectively.
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