Journal
MICROCHIMICA ACTA
Volume 186, Issue 5, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3436-3
Keywords
Colorimetric aptasensor; Hexadecyl trimethyl ammonium ion; Peroxidase-like activity; Malachite green; Gold nanoparticles; Aquaculture water
Categories
Funding
- Foundation from the Science and Technology Planning Project of Fujian Province, China [2016Y0064]
- Natural Science Foundation of Fujian Province of China [2018 J01432, 2017 J01633]
- National Key R and D Program of China [2018YFD0901003]
- Science and Technology Planning Project of Xiamen, China [3502Z20183031]
- National Undergraduate Training Programs for Innovation and Entrepreneurship [201610390042, 201710390022, 201810390071, 20181xj008]
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A specific and sensitive colorimetric aptasensor is described for the determination of Malachite Green (MG). It is exploiting the inhibition of the peroxidase-like activity of gold nanoparticles (AuNPs). The AuNPs act as enzyme mimics that catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to yield a dark blue solution. The catalytic activity is inhibited by hexadecyl trimethyl ammonium ion, specifically by cetyltrimethylammonium bromide (CTAB), which causes the aggregation of AuNPs. If a (negatively charged) RNA-aptamer against MG is added, it binds to the positively charged CTAB and prevents aggregation. This enhances the enzyme mimicking activity of the AuNPs and leads to the formation of a dark blue solution. However, in the presence of MG, the aptamer binds to MG, and leads to the aggregation of AuNPs again. The aggregated AuNPs possess a light blue color. A colorimetric method (best performed at 650nm) was work out that can detect MG in a concentration range from 10 to 500nmolL(-1). The detection limit based on 3 sigma/k criterion is 1.8nmolL(-1). The assay is highly specific and accurate. Recoveries from spiked real samples (aquaculture water) ranged from 80% to 120%.
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