Journal
MICROCHIMICA ACTA
Volume 186, Issue 5, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-019-3381-1
Keywords
Fluorometric assay; Nanoprobe; Hydrogen peroxide; Manganese dioxide
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Funding
- National Natural Science Foundation of China [21675001]
- Anhui Provincial Natural Science Foundation [1608085 MB46, 1608085MC67]
- Anhui Provincial Education Department Natural Sciences Key Fund [KJ2016SD23]
- Key Program in the Youth Elite Support Plan in Universities of Anhui Province [gxyqZD2016023]
- Provincial Project of Natural Science Re-search for Colleges and Universities of Anhui Province of China [KJ2016A274]
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A fluorescent probe for H2O2 is described. It is composed of MnO2 nanosheets and 5-carboxyfluorescein and was characterized by fluorescence, transmission electron microscopy, ultraviolet-visible absorption spectra, energy dispersive X-ray and Fourier transform infrared spectroscopy. The probe, with fluorescence excitation/emission maxima at 490/518nm, responds to H2O2 in the 1 to 200M concentration range and has a 0.33M detection limit. The probe was used in enzymatic assays for glucose and cholesterol by using the respective oxidases which produce H2O2. Responses are linear in the concentration range from 0.5 to 200M in case of glucose, and from 1 to 300M in case of cholesterol. The method was applied to quantify glucose and cholesterol in (spiked) serum samples.
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