Journal
METHODS
Volume 158, Issue -, Pages 22-26Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2019.02.007
Keywords
Bio-Plex; Mouse brain; Cerebral Malaria; Plasmodium berghei; Chemokine; Cytokine
Funding
- Intramural Research Program of the National Institute of Neurological Disorders and Stroke (NINDS), National Institutes of Health (NIH)
- Bio-Rad Laboratories
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [ZIANS003112] Funding Source: NIH RePORTER
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This protocol describes how to prepare mouse brain tissue for quantification of multiple inflammatory mediators using a multiplex bead-based immunoassay. It is important to have methods that allow quantification of multiple analytes from small amounts of tissue. Bio-Plex is a Luminex xMAP-based multiplex bead-based immunoassay technology that permits simultaneous analysis of up to 100 analytes from a single tissue sample. This assay has been used extensively to investigate analytes in plasma and serum samples as well as cultured and primary cells. Here, we describe a method for simultaneous analysis of 33 different inflammatory cytokines and chemokines from mouse brain tissue using the Bio-Plex Pro Mouse Chemokine Panel 33-Plex.
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