Journal
JOURNAL OF NEUROSCIENCE
Volume 39, Issue 24, Pages 4797-4813Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.0839-18.2019
Keywords
auditory brainstem response; AUT2; fragile X; high- and low-threshold potassium channels; medial nucleus of the trapezoid body; potassium channels
Categories
Funding
- Autifony Therapeutics Limited
- FRAXA Research Foundation
- National Institutes of Health [DC-01919, DC-000273, DC-008130, DC-016318]
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Fragile X syndrome (FXS) is characterized by hypersensitivity to sensory stimuli, including environmental sounds. We compared the auditory brainstem response (ABR) recorded in vivo in mice lacking the gene (Fmr1(-/y)) for fragile X mental retardation protein (FMRP) with that in wild-type animals. Wefound thatABRwave I, which represents input from the auditory nerve, is reduced in Fmr1(-/y) animals, but only at high sound levels. In contrast, wave IV, which represents the activity of auditory brainstem nuclei is enhanced at all sound levels, suggesting that loss of FMRP alters the central processing of auditory signals. Current-clamp recordings of neurons in the medial nucleus of the trapezoid body in the auditory brainstem revealed that, in contrast to neurons from wild-type animals, sustained depolarization triggers repetitive firing rather than a single action potential. In voltage-clamp recordings, K+ currents that activate at positive potentials (high-threshold K+ currents), which are required for high-frequency firing and are carried primarily by Kv3.1 channels, are elevated in Fmr1(-/y) mice, while K+ currents that activate near the resting potential and inhibit repetitive firing are reduced. Wetherefore tested the effects of AUT2 [((4-({5-[(4R)-4-ethyl-2,5-dioxo-1-imidazolidinyl]-2-pyridinyl} oxy)-2-(1-methylethyl) benzonitrile], a compound that modulates Kv3.1 channels. AUT2 reduced the high-threshold K+ current and increased the low-threshold K+ currents in neurons fromFmr1(-/y) animals by shifting the activation of the high-threshold current tomorenegative potentials. This reduced the firing rate and, in vivo, restored wave IV of the ABR. Our results from animals of both sexes suggest that the modulation of the Kv3.1 channel may have potential for the treatment of sensory hypersensitivity in patients with FXS.
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