Journal
JOURNAL OF NEUROIMMUNOLOGY
Volume 330, Issue -, Pages 143-151Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.jneuroim.2019.03.009
Keywords
Hippocampus kuda; Anti-inflammation; 2 '-Hydroxy-5 '-Methoxyacetophenone; NF-Kb; Molecular docking
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Funding
- Yangfan Scarce Top Talent Project of Guangdong Province [201433009]
- Program for Scientific Research Start-Up Funds of Guangdong Ocean University
- Development Project about Marine Economy Demonstration of Zhanjiang City [2017C8B1]
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Seahorse has been used as a traditional medicine in Southeast Asian countries for a long time. A compound, 2'-Hydroxy-5'-Methoxyacetophenone (2H5M) isolated from seahorse, Hippocampus kuda, was tested for its anti-inflammatory effect in lipopolysaccharides (LPS)-stimulated BV-2 cells and RAW264.7 cells. MTT assay indicated that 2H5M has no cytotoxicity on two kinds of cells. The concentration of nitric oxide (NO) measured by Griess Reaction System showed that 2H5M could significantly inhibit the NO concentration. The ELISA results showed that 2H5M could suppress the secretion of TNF-alpha in a dose-dependent manner. Moreover, western blot analysis was utilized to measure the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kappa B) signaling pathways. Electrophoretic mobility shift assay (EMSA) demonstrated that 2H5M reduced NF-kappa B DNA binding activity. Furthermore, the molecular docking study showed that 2H5M can form active sites with NF-kappa B. Collectively, these results indicated that 2H5M possesses anti-inflammatory effects and may have a potential application in inflammatory disorders in the future.
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