Journal
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
Volume 67, Issue 7, Pages 511-522Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1369/0022155419845373
Keywords
Decorin cDNA; ErbB2; HER2; oncosuppressive; transduction; vulva carcinoma
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Funding
- fund of Varsinais-Suomi Health District
- Cancer Society of Southwestern Finland
- Swedish Cultural Foundation in Finland
- Waldemar von Frenckell Foundation
- Medical Foundation for Life and Health in Finland
- Finnish-Norwegian Medical foundation
- Jane and Aatos Erkko Foundation [170046]
- fund of Satakunta Central Hospital
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The extracellular matrix proteoglycan decorin is well-known for its oncosuppressive activity. Here, decorin expression was examined in human vulva carcinoma tissue samples and in primary and commercial cell lines representing this malignant disease. Furthermore, the effect of adenovirus-mediated decorin cDNA (Ad-DCN) transduction on the viability, proliferation, and the expression and activity of the epidermal growth factor receptor (ErbB/HER) family members of the cell lines were investigated. Using in situ hybridization and immunohistochemistry for decorin, it was demonstrated that malignant cells in human vulva carcinoma tissues lack decorin expression. This result was true independently on tumor stage, grade or human papillomavirus status. RT-qPCR analyses showed that the human vulva carcinoma cell lines used in this study were also negative for decorin expression. Transduction of the cell lines with Ad-DCN caused a marked reduction in cell viability, while the proliferation of the cells was not affected. Experiments examining potential mechanisms behind the oncosuppressive effect of Ad-DCN transduction revealed that ErbB2/HER2 expression and activity in carcinoma cells were markedly downregulated. In conclusion, the results of this study showed that human vulva carcinoma cells lack decorin expression, and that Ad-DCN transduction of these cells induces oncosuppressive activity in part via downregulation of ErbB2/HER2.
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