Journal
JOURNAL OF ESSENTIAL OIL RESEARCH
Volume 31, Issue 5, Pages 361-378Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10412905.2019.1611671
Keywords
Litsea cubeba; essential oil; citral; d-limonene; antimicrobial activity; minimal inhibitory concentrations
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There are two chemotypes of Litsea cubeba (Lour.) Pers. (fam. Lauraceae) essential oils (EOs), either dominated by citral and limonene or by citronellal. L. cubeba EOs exhibits broad antimicrobial activity against bacteria and fungi with minimal inhibitory concentrations (MICs) from 0.5 to 2500 mu g/mL. This antimicrobial activity is attributed to the monoterpene citral, exhibiting a broad antimicrobial spectrum against bacteria and fungi (MICs 50-1000 mu g/mL). Pseudomonas aeruginosa were found most resistant, with MIC values above 1000 mu g/mL. Citral's mode of action is not fully investigated. At biocidal concentrations, it affects membrane structure and cell respiration, leading to rapid energy depletion and cell death. Involvement of intracellular reactive oxygen species has not been verified. Limonene exhibits insignificant antimicrobial activity. MICs of d-limonene range from 1 to 20 mg/mL. Citral and L. cubeba EOs were also subjects of food application studies. Promising results have been reported for fresh produce or fruit-juice.
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