Journal
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
Volume 54, Issue 6, Pages 533-537Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/03601234.2019.1586034
Keywords
Quercetin; ovarian functions; progesterone; 17 beta-estradiol; proliferation; apoptosis
Funding
- Ministry of Education, Science, Research and Sports of the Slovak Republic projects [VEGA 1/0039/16, KEGA 011SPU-4/2016, APVV-16-0170, APVV-15-0543]
- excellent scientific team Center of Animal Reproduction (CeRA)
- European Commission [26220220180]
- Tatra Bank Foundation
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Quercetin is a dietary bioflavonoid used widely as a food supplement and is generally recognized as safe. The aim of this in vitro study was to examine the steroid hormone (progesterone and 17- beta estradiol) release, proliferation (PCNA and cyclin B1) and apoptosis (caspase 3 and p53) of porcine ovarian granulosa cells after the addition of quercetin at concentrations 0.01, 0.1, 1, 10 and 100 mu mol L-1. Progesterone release was stimulated at the concentration 10 mu mol L-1. Quercetin neither had any impact on 17-beta estradiol secretion nor on the presence of PCNA. However, a significant enhancement of the occurrence of cyclin B1 was noted except for the lowest concentration 0.01 mu mol L-1. Quercetin did not have any influence on the number of granulosa cells containing caspase 3, but at the concentration 10 mu mol L-1 it inhibited p53 occurrence. Results confirm the safety of quercetin in porcine ovarian granulosa cell model and further suggest its possible concentration-dependent influence on ovarian functions through pathway that may involve progesterone, cyclin B1 and p53.
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