4.5 Article

In vitro cell uptake evaluation of curcumin-loaded PCL/F68 nanoparticles for potential application in neuronal diseases

Journal

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Volume 52, Issue -, Pages 905-914

Publisher

ELSEVIER
DOI: 10.1016/j.jddst.2019.05.042

Keywords

Nanoparticles; Neuronal diseases; Polycaprolactone; Curcumin; Pluronic (R) F68

Funding

  1. DGAPA
  2. UNAM
  3. DGAPA [PAPIIT TA 200318, IT1000117]
  4. CONACYT [A1-S-15759, CB 258043]
  5. Program 127 Formacion basica en investigacion (UNAM)
  6. [PAPIIT 2019143]
  7. [CONACYT CB 221629]
  8. [CONACYT INFRA 25194]
  9. [PIAPI 001]

Ask authors/readers for more resources

Curcumin is the major polyphenolic compound of Curcuma longa. It has been found to possess anti-inflammatory and antioxidant activities. The latter property could be exploited as a treatment for neuronal diseases. However, its medical application is limited due to its poor solubility in aqueous phase and fast degradation. Nanoparticle-based drug-delivery platforms are suitable carriers for overcoming pharmacokinetic limitations and enhancing drug transport, and they exhibit minimal toxicity. The purpose of this article was to assemble curcumin-loaded poly-epsilon-caprolactone nanoparticles by means of the emulsification-diffusion method using Pluronic (R) F68 as a stabilizer for in vitro evaluation in MIO-M1 and SH-SY5Y cells, which are cells involved on neuronal diseases. The curcumin-loaded nanoparticles obtained, with a mean particle size of 149.0 +/- 2.2 nm, were characterized by physicochemical and biological techniques. Results showed that curcumin was loaded with 96% efficacy. Additionally, the cytotoxicity of the nanoparticles was evaluated in vitro in MIO-M1 and SH-SY5Y cells, demonstrating adequate biocompatibility. Cell uptake was analyzed with MIO-M1 cells, exhibiting high internalization rate. These results indicate that curcumin-loaded nanoparticles could be a potential alternative treatment for neuronal diseases as spinocerebellar ataxias and Parkinson's disease.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available