Journal
JOURNAL OF CLINICAL INVESTIGATION
Volume 129, Issue 6, Pages 2578-2594Publisher
AMER SOC CLINICAL INVESTIGATION INC
DOI: 10.1172/JCI98857
Keywords
-
Categories
Funding
- NIH [AR 071432]
Ask authors/readers for more resources
Cortical bones account for more than 80% of human bone mass. The periosteum, a thin tissue that covers almost the entire bone surface, is essential for bone formation and regeneration. However, its osteogenic and bone regenerative abilities are not well studied. In this study, we found that macrophage-lineage cells recruit periosteum-derived cells (PDCs) for cortical bone formation. Knockout of colony-stimulating factor-1 eliminated macrophage-lineage cells and resulted in loss of PDCs with impaired periosteal bone formation. Moreover, macrophage-lineage tartrate-resistant acid phosphatase-positive (TRAP(+)) cells induced transcriptional expression of periostin and recruitment of PDCs to the periosteal surface through secretion of PDGF-BB, where the recruited PDCs underwent osteoblast differentiation coupled with type H vessel formation. We also found that subsets of Nestin(+) and LepR(+)CD45-Ter119-CD31-cells (LepR(+) PDCs) possess multipotent and self-renewal abilities and contribute to cortical bone formation. Nestin(+) PDCs are found primarily during bone development, whereas LepR(+) PDCs are essential for bone homeostasis in adult mice. Importantly, conditional knockout of Pdgfr-beta in LepR(+) cells impaired periosteal bone formation and regeneration. These findings uncover the essential role of periosteal macrophage-lineage cells in regulating periosteum homeostasis and regeneration.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available