Journal
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
Volume 1124, Issue -, Pages 1-6Publisher
ELSEVIER
DOI: 10.1016/j.jchromb.2019.05.024
Keywords
Molecular imprinting polymers; Nanoparticles; Affinity chromatography; Solid-phase synthesis; Antibodies; Protein epitope
Funding
- Wellcome Trust (UK)
- FONDECYT, Chile [11160506, 3170874]
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Herein an approach to prepare molecularly imprinted polymer nanoparticles (nanoMlPs) with specific binding affinity for antibodies is reported. The process relied on the covalent immobilization of the template (whole immunoglobulin G (IgG), Fc domain of human IgG and peptide epitope) onto the surface of a solid support, polymerization and affinity separation of nanoMlPs. The binding between nanoMIPs and their corresponding templates was analyzed and evaluated as being in sub-nanomolar and nano-molar range. The nanoMlPs prepared for Fc domain and epitope demonstrated a specific recognition of both human and goat IgGs, therefore they could be considered as a synthetic analogue of protein A and benefit from its intrinsic stability, short time and low cost of preparation.
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