Co-inoculation of Bacillus sp. and Pseudomonas putida at different development stages acts as a biostimulant to promote growth, yield and nutrient uptake of tomato

Aims This study builds upon the premise that roots culture distinct bacteria at specific stages of plant growth to benefit of specific microbial services needed at that particular growth stage. Accordingly, we hypothesized that the co-inoculation of beneficial microbes with distinct properties at specific stages of plant development would enhance plant performance. Methods and Results The chosen microbes were Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus mojavensis and Pseudomonas putida. These microbes were selected based on their specific services ranging from nutrient solubilization, root growth promotion and disease resistance, and were applied to the roots of tomato plants at specific time points when those services were needed the most by the plant. Laboratory and greenhouse studies were conducted to evaluate the effects of co-inoculation at specific stages of development compared to single microbial applications. Conclusion In general, the combination of three microbes gave the highest biomass and yield without the presence of disease. Applications of three microbes showed the highest root/shoot ratio, and applications of four microbes the lowest ratio. Pseudomonas putida significantly increased fruit macronutrient and micronutrient contents. Significance and Impact of the Study Our studies suggest that co-inoculation of three or four microbes is a good strategy for healthy crop production.