Meta-regression analysis to predict the influence of branched-chain and large neutral amino acids on growth performance of pigs

A meta-analysis was conducted to evaluate the effects of branched-chain amino acids (BCAA), their interactions, and interactions with large neutral amino acids (LNAA) to develop prediction equations for growth performance of pigs. Data from 25 papers, published from 1995 to 2018, for a total of 44 trials and 210 observations were recorded in a database. Diets were reformulated using the NRC (2012) loading values to estimate nutrient concentrations. The response variables were average daily gain (ADG), average daily feed intake (ADFI), and gain-to-feed ratio (G:F). The predictor variables tested included average body weight (BW), crude protein, neutral detergent fiber, Ile:Lys, Leu:Lys, Val:Lys, BCAA:Lys, Ile:Leu, Val:Leu, Ile:Val, (Ile+Val):Leu, Trp:Lys, Leu:Trp, Ile:Trp, Val:Trp, BCAA:Trp, Met:Lys, Leu:Met, Ile:Met, Val:Met, BCAA:Met, His:Lys, Leu:His, Ile:His, Val:His, BCAA:His, Thr:Lys, Leu:Thr, Ile:Thr, Val:Thr, BCAA:Thr, (Phe+Tyr):Lys, Leu:(Phe+Tyr), Ile:(Phe+Tyr), Val:(Phe+Tyr), BCAA:(Phe+Tyr), LNAA:Lys, Leu:LNAA, Ile:LNAA, Val:LNAA, and BCAA:LNAA. Amino acids were expressed on standardized ileal digestible basis. The MIXED procedure of SAS (SAS Institute Inc., Cary, NC) was used to develop the equations. The inverse of squared SEM was used to account for heterogeneous errors using the WEIGHT statement. Models were selected with a step-wise manual forward selection. In order to be included in the final model, predictor variables had to be statistically significant (P < 0.05) and provide an improvement of at least 2 points in Bayesian information criterion. The optimum equations were: ADG, g = - 985.94 + (15.2499 x average BW (kg)) - (0.08885 x average BW x average BW) + (1.063 x Leu:Lys) + (20.2659 x Ile:Lys) - (0.1479 x Ile:Lys x Ile:Lys) + (9.2243 x (Ile+Val):Leu) - (0.03321 x (Ile+Val):Leu x (Ile+Val):Leu) - (0.4413 x Ile:Trp); G:F, g/kg = 648.3 - (6.2974 x average BW (kg)) + (0.02051 x average BW x average BW) + (0.5396 x Ile:Lys) + (1.7284 x Val:Lys) - (0.00795 x Val:Lys x Val:Lys) - (1.7594 x Met:Lys); and ADFI, kg = predicted ADG/predicted G:F. Overall, the prediction equations suggest that increasing Leu:Lys negatively impacts ADG due to a reduction in G:F and ADFI caused by insufficient levels of other BCAA and LNAA relative to Leu. According to the model, the addition of Val, Ile, and Trp, alone or in combination, has the potential to counteract the negative effects of high dietary Leu concentrations on growth performance.