Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 126, Issue -, Pages 891-898Publisher
ELSEVIER
DOI: 10.1016/j.ijbiomac.2018.12.238
Keywords
Pancreatic cancer; Long non-coding RNA highly up-regulated in liver cancer; MicroRNA-15a; Tumor growth; Tumor metastasis; PI3K/AKT pathway
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Pancreatic cancer is a common and lethal cancer in digestive system. This study investigated the potential oncogenic effects of IncRNA HULC on pancreatic cancer. Briefly, qRT-PCR was conducted to measure the expression of HULC and miR-15a in pancreatic tissues and cells. Cell transfection was used to change the expression of HULC and miR-15a in pancreatic cancer Panc-1 cells. The viability, migration and invasion and apoptosis of Panc-1 cells after relevant transfection were detected using CCK-8 assay, two chamber transwell assay and Guava Nexin assay, respectively. Results found that HULC had a higher expression level, while miR-15a had a lower expression level in pancreatic cancer tissues. Overexpression of HULC promoted the proliferation, migration and invasion of Panc-1 cells. Suppression of HULC had opposite effects and dramatically induced cell apoptosis. Moreover, HULC negatively regulated the expression of miR-15a in Panc-1 cells. miR-15a participated in the effects of HULC on Panc-1 cells. Furthermore, overexpression of HULC activated PI3K/AKT pathway in Panc-1 cells by down-regulating miR-15a. In conclusion, HULC exerted oncogenic role in pancreatic cancer. Overexpression of HULC promoted the proliferation, migration and invasion of pancreatic cancer cells by down-regulating miR-15a and then activating PI3K/AKT pathway. (C) 2019 Elsevier B.V. All rights reserved.
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