Journal
FOOD CHEMISTRY
Volume 302, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2019.04.022
Keywords
Surface plasmon resonance biosensor; 3-Methyl-quinoxaline-2-carboxylic acid; Olaquindox; Residues; Swine tissues
Funding
- Ministry of Science and Technology of the People's Republic of China [2011DFA32140]
- National Natural Science Foundation of China [31772074]
- Fundamental Research Funds for the Central Universities [2662017PY049, 2017BC010]
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To monitor the illegal use of olaquindox in animals, a monoclonal antibody-based surface plasmon resonance (SPR) biosensor method has been developed to detect 3-methyl-quinoxaline-2-carboxylic acid, the marker residues of olaquindox, in swine tissues. The limit of detection was 1.4 mu g kg(-1) in swine muscle and 2.7 mu g kg(-1) in swine liver, which are lower than the EU recommended concentration (10 mu g kg(-1)). The recoveries were from 82% to 104.6%, with coefficients of variation of less than 12.2%. Good correlations between SPR and HPLC results (r = 0.9806, muscle; r = 0.9698, liver) and between SPR and ic-ELISA results (r = 0.9918, muscle; r = 0.9873, liver) were observed in the affected tissues, which demonstrated the reliability of the SPR method. This method would be a rapid and reliable tool for the screening of the residues of olaquindox in the edible tissues of animals.
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